Journal: Frontiers in Microbiology

Article Title: Local Bacteriophage Delivery for Treatment and Prevention of Bacterial Infections

doi: 10.3389/fmicb.2020.538060

Figure Lengend Snippet: Overview of biopolymers used for bacteriophage embedding.

Article Snippet: Phage delivery for internal medicine , Liposomes containing: ∙ DSCP phospholipids ∙ Cholesterol , S. aureus phage K ATCC 1985-B1 and E. coli phage ATCC 11303-B3 , In vitro , Liposomes were fabricated using microfluidic hydrodynamic flow focusing, with a phage load of ∼10 8 PFU/mL , Encapsulation in liposomes offered limited protection against acidic environments, with a 3log 10 reduction of phage. , Not assessed , .

Techniques: Viscosity, In Vitro, Incubation, Control, Preserving, In Vivo, Ex Vivo, Bacteria, CRISPR, Modification, Cream

Journal: Frontiers in Microbiology

Article Title: Local Bacteriophage Delivery for Treatment and Prevention of Bacterial Infections

doi: 10.3389/fmicb.2020.538060

Figure Lengend Snippet: Overview of the use of synthetic polymers for bacteriophage embedding.

Article Snippet: Phage delivery for internal medicine , Liposomes containing: ∙ DSCP phospholipids ∙ Cholesterol , S. aureus phage K ATCC 1985-B1 and E. coli phage ATCC 11303-B3 , In vitro , Liposomes were fabricated using microfluidic hydrodynamic flow focusing, with a phage load of ∼10 8 PFU/mL , Encapsulation in liposomes offered limited protection against acidic environments, with a 3log 10 reduction of phage. , Not assessed , .

Techniques: In Vitro, Encapsulation, Preserving, Isolation, Activity Assay, In Vivo, Dispersion, Incubation, Infection, Formulation, Control, Adhesive

Journal: Frontiers in Microbiology

Article Title: Local Bacteriophage Delivery for Treatment and Prevention of Bacterial Infections

doi: 10.3389/fmicb.2020.538060

Figure Lengend Snippet: Overview of the use of liposomes for bacteriophage encapsulation.

Article Snippet: Phage delivery for internal medicine , Liposomes containing: ∙ DSCP phospholipids ∙ Cholesterol , S. aureus phage K ATCC 1985-B1 and E. coli phage ATCC 11303-B3 , In vitro , Liposomes were fabricated using microfluidic hydrodynamic flow focusing, with a phage load of ∼10 8 PFU/mL , Encapsulation in liposomes offered limited protection against acidic environments, with a 3log 10 reduction of phage. , Not assessed , .

Techniques: Liposomes, Encapsulation, In Vitro, Homogenization, In Vivo, Control, Infection, Labeling, Marker, Bacteria, Emulsion, Confocal Microscopy

Journal:

Article Title: Complete Genomic Sequence of Bacteriophage B3, a Mu-Like Phage of Pseudomonas aeruginosa †

doi: 10.1128/JB.186.19.6560-6574.2004

Figure Lengend Snippet: Phages, prophages, and phage-related elements with homology to phage B3 a

Article Snippet: Bacteriophage B3 (ATCC 15692-B1) and the host bacterium, P. aeruginosa strain PAO1 (ATCC 15692), were obtained from the American Type Culture Collection (Manassas, Va.).

Techniques:

Journal:

Article Title: Complete Genomic Sequence of Bacteriophage B3, a Mu-Like Phage of Pseudomonas aeruginosa †

doi: 10.1128/JB.186.19.6560-6574.2004

Figure Lengend Snippet: Comparison of the genome organization for phages B3 and Mu. The genomes of B3 and Mu are shown as thick lines and are drawn approximately to scale, with tick marks at 1-kb intervals. ORFs are shown as boxes; their positions were derived from GenBank accession numbers AF083977 and NC_000929 for the Mu genome and this work for B3 (GenBank accession number AF232233). The direction of transcription is indicated by an arrow; in addition, genes transcribed rightward are shown above the line, and those transcribed leftward are positioned below the line. Relevant Mu genes are identified by their gene names or sequential genome gp numbers, and functions if known. The predicted B3 gene functions shown were deduced from the known functions of homologous genes in Mu or other phages; for B3 genes with Mu homologues, the identity of the homologous Mu gene is also given.

Article Snippet: Bacteriophage B3 (ATCC 15692-B1) and the host bacterium, P. aeruginosa strain PAO1 (ATCC 15692), were obtained from the American Type Culture Collection (Manassas, Va.).

Techniques: Derivative Assay

Journal:

Article Title: Complete Genomic Sequence of Bacteriophage B3, a Mu-Like Phage of Pseudomonas aeruginosa †

doi: 10.1128/JB.186.19.6560-6574.2004

Figure Lengend Snippet: Diagrammatic representation of the degree of relatedness for B3 genes with homologues in other Mu-like phages and prophages. The B3 map, ORFs, and predicted protein functions are shown as in Fig. ​Fig.1,1, except that all ORFs are shown above the kilobase line. Phages and prophages with homology to at least six B3 genes are included, and their names are listed on the left. Horizontal lines drawn below a B3 gene indicate the presence of a homologue in the corresponding Mu-like phage or prophage. The thickness of the line indicates the degree of similarity, achieved by dividing the BLAST expect values in Table S2 in the supplemental material into five groups, with the thickest line representing the greatest similarity (group I) and decreasing thickness indicating decreasing similarity. The expect value boundaries for the five groups are as follows: e-50 or better for group I, e-49 to e-15 for group II, e-14 to e-4 for group III, 0.001 to 5.0 for group IV, and >5.0 for group V on PSI-BLAST iteration 1 but 1.0 or better on PSI-BLAST iteration 2.

Article Snippet: Bacteriophage B3 (ATCC 15692-B1) and the host bacterium, P. aeruginosa strain PAO1 (ATCC 15692), were obtained from the American Type Culture Collection (Manassas, Va.).

Techniques: